Government Online has several thousand users in the public sector. These include government departments, executive agencies, local councils, schools, colleges and universities, and many other related organisations. Among the most well known are the following:

Audit Commission
Bexley Council
Bolton Metropolitan Council
Bracknell Forest Borough Council
Caerphilly County Borough Council
Dacorum District Council
Department of Environment, Food and Rural Affairs
Defence Science and Technology Laboratory
Devon County Council
Doncaster Metropolitan Borough Council
East Riding of Yorkshire Council
Estyn (HM Inspectorate For Education and Training in Wales)
Foreign and Commonwealth Office
Improvement and Development Agency
Institute of Food Research
Leicester City Council
Liverpool University
Manchester City Council
Ministry of Defence
National College for School Leadership
National School of Government
National Museum of Science and Industry
Natural Environment Research Council
Newcastle City Council
Northampton Borough Council
Nottinghamshire County Council
OGCBuying.Solutions
Oxfordshire County Council
Perth and Kinross Council
Sedgemoor Borough Council
St Helens Council
Stockport Metropolitan Borough Council
Thames Valley Police
Uttlesford District Council